ProSci

Tim-3 Antibody [2C12], Rabbit IgG

Product Code:
 
PSI-24-615
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
2C12
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Applications:
  • Blocking
  • Flow Cytometry
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Flow cytometry using the Anti-Tim-3 antibody 2C12 ( </b> Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of 2C12 (24-615- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
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<b> ELISA of anti-TIM-3 antibody on TIM-3-Fc fusion protein.. </b> Binding curves of the rmeric version of the anti-TIM-3 antibody 2C12 (24-615; blue line) and isotype control (3.0- anti-Fluorescein; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein (Pr00228-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using 24-615. For signal detection- anti-r1 HRP (tibody was used.
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<b> Flow cytometry using the Anti-Tim-3 antibody 2C12 ( </b> Paraformaldehyde fixed mouse splenocytes permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control- black line) or the r version of 2C12 (24-615- blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
<b> ELISA of anti-TIM-3 antibody on TIM-3-Fc fusion protein.. </b> Binding curves of the rmeric version of the anti-TIM-3 antibody 2C12 (24-615; blue line) and isotype control (3.0- anti-Fluorescein; red line) to an ELISA plate coated with mouse TIM-3-Fc fusion protein (Pr00228-1.9) at a concentration of 5 ug/ml. A 3-fold serial dilution from 3000 to 0.0169 ng/ml was performed using 24-615. For signal detection- anti-r1 HRP (tibody was used.

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CodeSizePrice
PSI-24-615-0.2mg0.2mg£681.00
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Further Information

Additional Names:
CD366, HAVcr-2, HAVCR2, Hepatitis A virus cellular receptor 2 homolog, T-cell immunoglobulin and mucin domain containing 3, T-cell immunoglobulin and mucin domain-containing protein 3, T-cell immunoglobulin mucin receptor 3, T-cell membrane protein 3, TIM-3, TIMD-3, TIM3
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund?s adjuvant.
NCBI Gene ID #:
171285
NCBI Official Name:
hepatitis A virus cellular receptor 2
NCBI Official Symbol:
Havcr2
NCBI Organism:
Mus musculus
Research Area:
Immunology, Cancer, Chemokines & Cytokines
Swissprot #:
Q8VIM0
User NOte:
For research use only.