ProSci

ED-B domain of fibronectin Antibody [A2], Rabbit IgG

Product Code:
 
PSI-24-455
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
A2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Frozen Section (IHC-F)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody A2 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of A2 (24-455) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing endoplasmic reticular staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-455- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.
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<b> Immunofluoresence staining of fixed HeLa cells with anti-ED-B domain of fibronectin antibody A2 (</b> Immunofluorescence analysis of paraformaldehyde fixed HeLa cells- permewith 0.15% Triton stained with the chimeric r version of A2 (24-455) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing endoplasmic reticular staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-455- DAPI- merged channels and a negative control. The negative control was stained with unimmunized r followed by Alexa Fluor®?488 secondary antibody.

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PSI-24-455-0.2mg0.2mg£681.00
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Further Information

Additional Names:
EDB(+) FN, Extra domain-B containing fibronectin
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This full-length, chimeric rabbit antibody was made using the variable domain sequences of the original Human scFv format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
2D-PAGE gel isolated human Extra-Domain containing fibronectin from lysate of human melanoma COLO-38 cells.
NCBI Gene ID #:
2335
NCBI Official Name:
fibronectin 1
NCBI Official Symbol:
FN1
NCBI Organism:
Homo sapiens
Research Area:
Other, Signal Transduction, Immunology, Cell Biology, Cancer
Swissprot #:
P02751
User NOte:
For research use only.