ProSci

IL2R Antibody [AHT107], Rabbit IgG

Product Code:
 
PSI-24-563
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AHT107
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Blocking
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Flow Cytometry
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Immunofluorescence staining of Jurkat cells using anti-IL2R antibody (AHT107. </b> Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of AHT107 (24-563) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-563- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.
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<b> Immunofluorescence staining of Jurkat cells using anti-IL2R antibody (AHT107. </b> Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of AHT107 (24-563) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-563- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.

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CodeSizePrice
PSI-24-563-0.2mg0.2mg£681.00
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Further Information

Additional Names:
CD25, Interleukin 2 receptor, IL-2R, IL-2 receptor, IL2 receptor, Tac antigen, p55, AHT-107, AHT107
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
BALB/c mice were immunized with human T lymphoblasts and spleen cells of the immunized animals were fused with X63-Ag8.653 mouse myeloma cells. The supernatants of the resultant hybridomas were screened by a proliferation inhibition assay (inhibition of the human T lymphoblast response to IL-2). Two of those (the clones AHT54 and AHT107) that produced antibodies which inhibited IL-2 dependent proliferation of human T lymphoblasts were selected, cloned and recloned.
NCBI Gene ID #:
3559
NCBI Official Name:
HGNC:HGNC:11920
NCBI Official Symbol:
FAS
NCBI Organism:
Homo sapiens
Research Area:
Infectious Disease, Immunology
Swissprot #:
P01589
User NOte:
For research use only.