ProSci

CD32 Antibody [AT130-5], Rabbit IgG

Product Code:
 
PSI-24-289
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
AT130-5
Regulatory Status:
 
RUO
Target Species:
 
Mouse
Application:
 
Immunohistochemistry (IHC)
Shipping:
 
Blue Ice
Storage:
 
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at -20⁰C.
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<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD32 antibody AT130-5 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse (<i>Mus musculus</i>) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of AT130-5 (24-289) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-289- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

<b> Immunofluorescence staining of fixed mouse splenocytes with anti-CD32 antibody AT130-5 (</b> Immunofluorescence analysis of paraformaldehyde fixed mouse (<i>Mus musculus</i>) splenocytes on Shi-fix™ coverslips- permewith 0.15% Triton stained with the chimeric rsion of AT130-5 (24-289) at 10 ug/ml for 1h followed by Alexa Fluor®?488 secondary antibody (1 ug/ml)- showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom 24-289- DAPI- merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody (0) followed by Alexa Fluor®?488 secondary antibody.

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PSI-24-289-0.2mg0.2mg£681.00
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Further Information

Additional Names:
FcgRIIb, Low affinity immunoglobulin gamma Fc region receptor II-b, FCGR2B
Buffer:
PBS with 0.02% Proclin 300.
Chimeric Use Statement:
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
CD32-rat CD4 domain 3/4 fustion protein.
NCBI Gene ID #:
14130
NCBI Official Name:
Fc receptor, IgG, low affinity Iib
NCBI Official Symbol:
Fcgr2b
NCBI Organism:
Mus musculus
Research Area:
Immunology, Signal Transduction
Swissprot #:
P08101
User NOte:
For research use only.