ProSci

IL-3 beta Antibody (biotin)

Product Code:
 
PSI-38-343
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Caprine (Goat)
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect Rat IL-3 beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IL-3 beta as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat IL-3 beta.
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To detect Rat IL-3 beta by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.25 ? 1.0 ug/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat IL-3 beta as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat IL-3 beta.

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CodeSizePrice
PSI-38-343-0.025mg0.025mg£505.00
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This product comes from: United States.
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Further Information

Additional Names:
MCGF (Mast cell growth factor), Multi-CSF, HCGF, P-cell stimulation factor
Application Note:
Sandwich ELISA: To detect Rat IL-3 beta by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 ? 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with ProSci?s Polyclonal Anti-Rat IL-3 beta as a capture antibody, allows the detection of at least 0.2 ? 0.4 ng/well of recombinant Rat IL-3 beta.
Western Blot
To detect Rat IL-3 beta by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat IL-3 beta is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-3 is a hematopoietic growth factor that promotes the survival, differentiation and proliferation of committed progenitor cells of the megakaryocyte, granulocyte-macrophage, erythroid, eosinophil, basophil and mast cell lineages. Produced by T cells, mast cells and eosinophils, IL-3 enhances thrombopoiesis, phagocytosis, and antibody-mediated cellular cytotoxicity. Its ability to activate monocytes suggests that IL-3 may have additional immunoregulatory roles. Many of the IL-3 activities depend upon co-stimulation with other cytokines. IL-3 is a species-specific, variably glycosylated cytokine. Recombinant Rat IL-3beta is a 16.3 kDa globular protein containing 144 amino acid residues.
Concentration:
batch dependent
Conjugate:
Biotin
Immunogen:
Produced from sera of goats pre-immunized with highly pure (>98%) recombinant Rat IL-3beta. Anti-Rat IL-3beta specific antibody was purified by affinity chromatography and then biotinylated.
NCBI Gene ID #:
24495
NCBI Official Name:
interleukin 3
NCBI Official Symbol:
Il3
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P97688
Protein GI Number:
P04823.1
Purification:
IL-3 beta specific antibody was purified by affinity chromatography and then biotinylated
Research Area:
Infectious Disease,Cancer,Other,Immunology,Cell Cycle,Stem Cell
Swissprot #:
P97688
User NOte:
Centrifuge vial prior to opening.