ProSci

IL-13 Antibody

Product Code:
 
PSI-38-344
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Western Blot (WB)
Shipping:
 
Blue Ice
Storage:
 
Antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
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To detect Rat IL-13 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Rat IL-13 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-13.
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To detect Rat IL-13 by sandwich ELISA (using 100ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci's Biotinylated Anti-Rat IL-13 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-13.

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CodeSizePrice
PSI-38-344-0.05mg0.05mg£505.00
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This product comes from: United States.
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Further Information

Additional Names:
NC300 (Human), P600 (Murine)
Application Note:
Sandwich ELISA: To detect Rat IL-13 by sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5 - 2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with ProSci?s Biotinylated Anti-Rat IL-13 as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Rat IL-13.
Western Blot
To detect Rat IL-13 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μg/ml. When used in conjunction with compatible secondary reagents, the detection limit for recombinant Rat IL-13 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Background:
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1b, TNF-a, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching, resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 are cross-species reactive. Recombinant Rat IL-13 a 12.3 kDa protein consisting of 113 amino acid residues.
Concentration:
batch dependent
Conjugate:
Unconjugated
Immunogen:
Produced from sera of rabbits immunized with highly pure recombinant Rat IL-13. Anti-Rat IL-13 specific antibody was purified by affinity chromatography employing an immobilized Rat IL-13 matrix.
NCBI Gene ID #:
116553
NCBI Official Name:
interleukin 13
NCBI Official Symbol:
Il13
NCBI Organism:
Rattus norvegicus
Physical State:
Lyophilized
Protein Accession #:
P42203
Protein GI Number:
P42203.1
Purification:
IL-13 specific antibody was purified by affinity chromatography employing an immobilized Rat IL-13 matrix
Research Area:
COVID-19,Infectious Disease,Cancer,Stem Cell,Immunology
Swissprot #:
P42203
User NOte:
Centrifuge vial prior to opening.