Anti-Human PD-1 (Cemiplimab)
Antibody Isotype:
Human IgG4κ
Antibody Clonality:
Monoclonal
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Flow Cytometry
- Functional Study
- Immunoprecipitation (IP)
- Western Blot (WB)
Shipping:
2 - 8°C Wet Ice
Storage:
Functional grade biosimilar antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage aseptically aliquot in working volumes without diluting and store at -80°C.?Avoid Repeated Freeze Thaw Cycles.
No additional charges, what you see is what you pay! *
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available!
Contact us to find what you can save.
This product comes from:
US.
Typical lead time:
14-21 working days.
Contact us for more accurate information.
- Further Information
- References
- Show All
Further Information
PD-1 is expressed on activated T cells, B cells, a subset of thymocytes, macrophages, dendritic cells, and some tumor cells and is also retained in the intracellular compartments of regulatory T cells (Tregs).
? 5.0 mg/ml
Purified No Carrier Protein
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
This biosimilar antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Human PD-1
PD-1 is a transmembrane protein in the CD28/CTLA-4 subfamily of the Ig superfamily1, 2. When stimulated via the T cell receptor (TCR), Tregs translocate PD-1 to the cell surface3. Programmed cell death 1 ligand 1 (PD-L1; CD274; B7H1) and programmed cell death 1 ligand 2 (PD-L2; CD273; B7DC) have been identified as PD-1 ligands1. PD-1 is co-expressed with PD-L1 on tumor cells and tumor-infiltrating antigen-presenting cells (APCs)2. Additionally, PD-1 is co-expressed with IL2RA on activated CD4+ T cells3.
PD-1 is an immune checkpoint receptor that suppresses cancer-specific immune responses4. Additionally, PD-1 acts as a T cell inhibitory receptor and plays a critical role in peripheral tolerance induction and autoimmune disease prevention as well as important roles in the survival of dendritic cells, macrophage phagocytosis, and tumor cell glycolysis2. PD-1 prevents uncontrolled T cell activity, leading to attenuation of T cell proliferation, cytokine production, and cytolytic activities. Additionally, the PD-1 pathway is a major mechanism of tumor immune evasion, and, as such, PD-1 is a target of cancer immunotherapy2.
Cemiplimab is a fully human, hinge-stabilized (S228P) high affinity anti-PD-1 antibody that potently blocks PD-1 interaction with PD-L1 and PD-L2 ligands and enhances human primary T-cell responses in vitro5. Cemiplimab was generated using VelocImmune knock-in mice immunized with recombinant human PD-1-mFc protein containing the PD-1 extracellular domain (amino acids 1-167) and the Fc portion of mouse IgG2a. Splenocyte-derived hybridomas were screened for human monoclonal antibody reactivity to recombinant human PD-1-hFc (extracellular domain of human PD-1 fused to human IgG1 Fc).
Cemiplimab is the first approved treatment in the United States and EU for patients with locally advanced or metastatic cutaneous squamous cell carcinoma who are not candidates for curative surgery or radiotherapy6.
5133
?95% by SDS Page, ?95% monomer by analytical SEC
PD-1
