Elabscience

Recombinant Fas Monoclonal Antibody

Product Code:
 
E-AB-81556
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
R02-8I7
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot detection of Fas in Hela,Jurkat cell lysates using Fas Rabbit mAb(1:500 diluted).Predicted band size:38kDa.Observed band size:45kDa.
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Immunohistochemistry of Fas in paraffin-embedded Human lung cancer tissue using Fas Rabbit mAb at dilution 1:50
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Western blot detection of Fas in Hela,Jurkat cell lysates using Fas Rabbit mAb(1:500 diluted).Predicted band size:38kDa.Observed band size:45kDa.
Immunohistochemistry of Fas in paraffin-embedded Human lung cancer tissue using Fas Rabbit mAb at dilution 1:50

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E-AB-81556-50uL50uL£184.00
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E-AB-81556-100uL100uL£251.00
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Further Information

Abbreviation:
Fas
Background:
FAS,also named as CD95,APO-1,APT1,FAS1 and TNFRSF6,is a receptor for TNFSF6/FASLG. It is a cell surface receptor belonging to the TNF receptor superfamily,can mediates apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains,leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance,in the antigen-stimulated suicide of mature T-cells,or both. The secreted isoforms 2 to 6 block apoptosis (in vitro). This anti-Fas monoclonal antibody can be used to induce apoptosis in cell cultures through Fas by imitating the Fas-ligand.
Buffer:
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Calculated MW:
38kDa
Concentration:
300 ug/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:1000 IHC 1:50-1:100
Immunogen:
A synthetic peptide of human Fas
ObservedMW:
45kDa
Purification method:
Affinity Purified
Target Synonym:
ALPS 1A;ALPS1A;APO 1;Apo 1 antigen;APO 1 cell surface antigen;Apo-1 antigen;APO1;Apo1 antigen;APO1 cell surface antigen;Apoptosis antigen 1;Apoptosis mediating surface antigen FAS;Apoptosis-mediating surface antigen FAS;APT 1;APT1;CD 95;CD 95 antigen;CD95;CD95 antigen;Delta Fas;Delta Fas/APO 1/CD95;Delta Fas/APO1/CD95;Fas (TNF receptor superfamily;member 6);FAS 1;FAS 827dupA;Fas AMA;Fas;FAS Antigen;Fas cell surface death receptor;FAS1;FASLG receptor;FASTM;sFAS;Surface antigen APO1;TNF receptor superfamily;member 6;TNFRSF 6;TNFRSF6;TNR6;Tumor necrosis factor receptor superfamily member 6
UNIProt ID:
P25445

Documents