Elabscience

Recombinant 4E BP1 Monoclonal Antibody

Product Code:
 
E-AB-81520
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
R06-8E6
Regulatory Status:
 
RUO
Target Species:
  • Hamster
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot detection of eIF4EBP1 in Jurkat,C6,CHO-K1 cell lysates using eIF4EBP1 Rabbit mAb(1:1000 diluted).Predicted band size:13kDa.Observed band size:15-20kDa.
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Immunohistochemistry of eIF4EBP1 in paraffin-embedded Human breast cancer tissue using eIF4EBP1 Rabbit mAb at dilution 1:50
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Western blot detection of eIF4EBP1 in Jurkat,C6,CHO-K1 cell lysates using eIF4EBP1 Rabbit mAb(1:1000 diluted).Predicted band size:13kDa.Observed band size:15-20kDa.
Immunohistochemistry of eIF4EBP1 in paraffin-embedded Human breast cancer tissue using eIF4EBP1 Rabbit mAb at dilution 1:50

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CodeSizePrice
E-AB-81520-50uL50uL£184.00
Quantity:
E-AB-81520-100uL100uL£251.00
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Further Information

Abbreviation:
4E BP1
Background:
Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1),is a member of 4EBPs family,which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E,thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors,hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1,alpha,beta and gamma.
Buffer:
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Calculated MW:
13kDa
Concentration:
300 ug/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:1000 IHC 1:50-1:100
Immunogen:
A synthetic peptide of human eIF4EBP1
ObservedMW:
15/20kDa
Purification method:
Affinity Purified
Target Synonym:
4E-BP1;4EBP1;4EBP1;BP 1;eIF4E binding protein 1;eIF4E-binding protein 1;Eif4ebp1;Eukaryotic translation initiation factor 4E-binding protein 1;PHAS-I;PHASI;Phosphorylated heat- and acid-stable protein regulated by insulin 1
UNIProt ID:
Q13541

Documents