Elabscience

Recombinant APG5L Monoclonal Antibody

Product Code:
 
E-AB-81459
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
R05-4F2
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot detection of APG5L/ATG5 Rabbit pA in K562,C6,Hela cell lysates using APG5L/ATG5 Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:55kDa.
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Immunofluorescence of APG5L/ATG5 (green) in MCF-7 using APG5L/ATG5 antibody at dilution 1:20, and DAPI(blue)
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Western blot detection of APG5L/ATG5 Rabbit pA in K562,C6,Hela cell lysates using APG5L/ATG5 Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:55kDa.
Immunofluorescence of APG5L/ATG5 (green) in MCF-7 using APG5L/ATG5 antibody at dilution 1:20, and DAPI(blue)

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CodeSizePrice
E-AB-81459-50uL50uL£184.00
Quantity:
E-AB-81459-100uL100uL£251.00
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Further Information

Abbreviation:
APG5L
Background:
ATG5,also named as APG5L and ASP,belongs to the ATG5 family. It is required for autophagy. It plays an important role in the apoptotic process,possibly within the modified cytoskeleton. Its expression is a relatively late event in the apoptotic process,occurring downstream of caspase activity. Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. It mediates autophagosome-independent host protection.
Buffer:
50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein
Calculated MW:
33kDa
Concentration:
300 ug/mL
Conjugation:
Unconjugated
Dilution:
WB 1:1000-1:2000 IF 1:20-1:100
Immunogen:
A synthetic peptide of human APG5L/ATG5
ObservedMW:
55kDa
Purification method:
Affinity Purified
Target Synonym:
APG 5;APG 5L;APG5;APG5 autophagy 5 like;APG5 like;APG5-like;APG5L;Apoptosis specific protein;Apoptosis-specific protein;ASP;ATG 5;Atg5;ATG5 autophagy related 5 homolog;ATG5;Autophagy protein 5;Autophagy related 5;hAPG5;Homolog of S Cerevisiae autophagy 5;OTTHUMP00000040507
UNIProt ID:
Q9H1Y0

Documents