Elabscience

C9orf72 Polyclonal Antibody

Product Code:
 
E-AB-91381
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
1 / 7
Western blot analysis of extracts of various cell lines using C9orf72 Polyclonal Antibody at 1:1000 dilution.
2 / 7
Western blot analysis of extracts of Rat brain using C9orf72 Polyclonal Antibody at 1:1000 dilution.
3 / 7
Immunohistochemistry of paraffin-embedded rat kidney using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
4 / 7
Immunohistochemistry of paraffin-embedded human esophageal cancer using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
5 / 7
Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
6 / 7
Immunofluorescence analysis of NIH/3T3 cells using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
7 / 7
Immunofluorescence analysis of U2OS cells using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines using C9orf72 Polyclonal Antibody at 1:1000 dilution.
Western blot analysis of extracts of Rat brain using C9orf72 Polyclonal Antibody at 1:1000 dilution.
Immunohistochemistry of paraffin-embedded rat kidney using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophageal cancer using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse kidney using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of NIH/3T3 cells using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using C9orf72 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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E-AB-91381-60uL60uL£210.00
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E-AB-91381-120uL120uL£291.00
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E-AB-91381-200uL200uL£431.00
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Further Information

Abbreviation:
C9orf72
Background:
The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.01% thiomersal,50% glycerol,pH7.3.
Calculated MW:
24kDa/54kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:100,IF 1:50-1:200
Immunogen:
Recombinant fusion protein of human C9orf72
ObservedMW:
50KDa
Purification method:
Affinity purification
Target Synonym:
C9orf72;ALSFTD;DENNL72;FTDALS;FTDALS1
UNIProt ID:
Q96LT7

Documents