Elabscience

BRD2 Polyclonal Antibody

Product Code:
 
E-AB-91481
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Western blot analysis of extracts of HeLa cells using BRD2 Polyclonal Antibody at 1:1000 dilution.HeLa cells were treated by NaCl (400mM)
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Immunohistochemistry of paraffin-embedded rat brain using BRD2 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Western blot analysis of extracts of HeLa cells using BRD2 Polyclonal Antibody at 1:1000 dilution.HeLa cells were treated by NaCl (400mM)
Immunohistochemistry of paraffin-embedded rat brain using BRD2 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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E-AB-91481-60uL60uL£210.00
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E-AB-91481-120uL120uL£291.00
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E-AB-91481-200uL200uL£431.00
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Further Information

Abbreviation:
BRD2
Background:
This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
74kDa/83kDa/88kDa/92kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human BRD2
ObservedMW:
110kDa
Purification method:
Affinity purification
Target Synonym:
BRD2;BRD2-IT1;D6S113E;FSH;FSRG1;NAT;O27.1.1;RING3;RNF3
UNIProt ID:
P25440

Documents