Elabscience

NKX6-1 Polyclonal Antibody

Product Code:
 
E-AB-92239
Product Group:
 
Primary Antibodies
Supplier:
 
Elabscience
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
Ice packs
Storage:
 
Store at -20°C. Avoid freeze/thaw cycles.
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Western blot analysis of extracts of various cell lines using NKX6-1 Polyclonal Antibody at 1:500 dilution.
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Immunohistochemistry of paraffin-embedded mouse pancreas using NKX6-1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Western blot analysis of extracts of various cell lines using NKX6-1 Polyclonal Antibody at 1:500 dilution.
Immunohistochemistry of paraffin-embedded mouse pancreas using NKX6-1 Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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CodeSizePrice
E-AB-92239-60uL60uL£210.00
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E-AB-92239-120uL120uL£291.00
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E-AB-92239-200uL200uL£431.00
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Further Information

Abbreviation:
NKX6-1
Background:
In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al.
Buffer:
PBS with 0.05% proclin300,50% glycerol,pH7.3.
Calculated MW:
37kDa
Concentration:
1mg/mL
Conjugation:
Unconjugated
Dilution:
WB 1:500-1:2000,IHC 1:50-1:200
Immunogen:
A synthetic peptide of human NKX6-1
ObservedMW:
45KDa/46KDa
Purification method:
Affinity purification
Target Synonym:
NKX6A; NKX6.1
UNIProt ID:
P78426

Documents