Recombinant Human MAPKAPK3 Protein (GST Tag)

MAPKAPK3

NP_004626.1

The MAPKAP kinases are a group of MAP kinase substrates which are themselves kinases. In response to activation, the MAP kinases phosphorylate downstream components on a consensus Pro-X-Ser/Thr-Pro motif. Several kinases that contain this motif have been identifed and serve as substrates for the ERK and p38 MAP kinases. Mitogen-activated protein (MAP) kinase-activated protein kinase 3, also known as MAPKAPK-3 and 3pK, is a member of the Ser/Thr protein kinase family. It is Widely expressed in human tissues, with a higher expression level observed in heart and skeletal muscle. No expression in brain. MAPKAPK-3 is unique since it was shown to be activated by three members of the MAPK family, namely extracellular-signal-regulated kinase (ERK), p38, and Jun-N-terminal kinase (JNK). It is highly activated both by mitogens and by stress-inducing agents or proinflammatory cytokines, and translocates to the cytoplasm from nucleus. MAPKAPK-3 is exclusively activated via the classical MAPK cascade, while stress-induced activation of MAPKAPK-3 is mainly mediated by p38, however the mechanism defining the specificity remains unknown.

69 kDa

< 1.0 EU per ug of the protein as determined by the LAL method.

Baculovirus-Insect Cells

Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 7.5, 0.25mM DTT, 0.1mM EDTA, 0.5mM PMSF, 10% glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

N-GST

69 kDa

> 90 % as determined by reducing SDS-PAGE.

Met 1-Gln 382

3PK;MAPKAP-K3;MAPKAP3;MAPKAPK-3;MK-3