NSJ Bioreagents

CD63 Antibody / LAMP-3

Product Code:
 
NSJ-V8875SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
LAMP3/4949
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the CD63 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
 

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CodeSizePrice
NSJ-V8875SAF-100UG100ug£534.00
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Further Information

Application Details:
ELISA: 1-5ug/ml (order BSA-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the CD63 antibody should be determined by the researcher.
Description:
CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Full-length human CD63 protein was used as the immunogen for the CD63 antibody.
Limitation:
This CD63 antibody is available for research use only.
Localization:
Cell surface, Cytoplasm
PM_Meta_Key:
Product_Images
PM_Meta_Value:
https://www.nsjbio.com/get_image.php?catalog_no=V8875-100UG&type=image1~FACS staining of bead-bound exosomes derived from MCF-7 cells: Unstained exosomes (gray) and exosomes stained with CD63 antibody (orange, clone LAMP3/4949).|https://www.nsjbio.com/get_image.php?catalog_no=V8875-100UG&type=image2~FACS staining of human MCF-7 cells with CD63 antibody (blue, clone LAMP3/4949), unstained cells (blue, clone gray).|https://www.nsjbio.com/get_image.php?catalog_no=V8875-100UG&type=image3~IHC staining of FFPE human tonsil tissue with CD63 antibody (clone LAMP3/4949) at 1ug/ml. Negative control inset: PBS instead of primary antibody to control for secondary binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.|https://www.nsjbio.com/get_image.php?catalog_no=V8875-100UG&type=image4~SDS-PAGE analysis of purified, BSA-free CD63 antibody (clone LAMP3/4949) as confirmation of integrity and purity.
Purity:
Protein A/G affinity
Uniprot #:
P08962

Documents