NSJ Bioreagents

Cytokeratin 8/18 Antibody Cocktail

Product Code:
 
NSJ-V8817SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SPM192 + SPM265
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Cytokeratin 8/18 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human lung carcinoma tissue with Cytokeratin 8/18 antibody (clone SPM192 + SPM265). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human lung carcinoma tissue with Cytokeratin 8/18 antibody (clone SPM192 + SPM265). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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NSJ-V8817SAF-100UG100ug£534.00
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Further Information

Application Details:
Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.
Description:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Keratin preparation from a human carcinoma (SPM192); PMC-42 human breast carcinoma cells (SPM265) was used as the immunogen for the Cytokeratin 8/18 antibody.
Limitation:
This Cytokeratin 8/18 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A/G affinity
Uniprot #:
P05787; P05783

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