NSJ Bioreagents

Clusterin Antibody / Apolipoprotein J / APO-J

Product Code:
 
NSJ-V9373SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CLU/4723
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the APO-J antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 4
IHC staining of FFPE human adrenal gland tissue with APO-J antibody (clone CLU/4723). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
Western blot analysis of human testis lysate using APO-J antibody (clone CLU/4723). Predicted molecular weight: 75-80 kDa (heterodimer precursor), 36-39 kDa (alpha subunit), 34-36 kDa (beta subunit).
3 / 4
SDS-PAGE analysis of purified, BSA-free APO-J antibody (clone CLU/4723) as confirmation of integrity and purity.
4 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using APO-J antibody (clone CLU/4723). These results demonstrate the foremost specificity of the CLU/4723 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
❮❯

IHC staining of FFPE human adrenal gland tissue with APO-J antibody (clone CLU/4723). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot analysis of human testis lysate using APO-J antibody (clone CLU/4723). Predicted molecular weight: 75-80 kDa (heterodimer precursor), 36-39 kDa (alpha subunit), 34-36 kDa (beta subunit).
SDS-PAGE analysis of purified, BSA-free APO-J antibody (clone CLU/4723) as confirmation of integrity and purity.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using APO-J antibody (clone CLU/4723). These results demonstrate the foremost specificity of the CLU/4723 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-V9373SAF-100UG100ug£534.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Application Details:
Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the APO-J antibody should be determined by the researcher.
Description:
Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. The beta subunit corresponds to residues 23-227. The alpha subunit corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 150-300 was used as the immunogen for the APO-J antibody.
Limitation:
This APO-J antibody is available for research use only.
Localization:
Nucleus, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P10909

Documents