NSJ Bioreagents

p120 Catenin Antibody / CTNND1 / Catenin delta 1

Product Code:
 
NSJ-V9163SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
CTNND1/4383R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the recombinant Delta 1 Catenin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
1 / 3
IHC staining of FFPE human colon tissue with recombinant Delta 1 Catenin antibody (clone CTNND1/4383R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 3
Negative tissue control. IHC analysis of formalin-fixed, paraffin-embedded human brain stained with CTNND1/4383R at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant Delta 1 Catenin antibody (CTNND1/4383R) as confirmation of integrity and purity.
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IHC staining of FFPE human colon tissue with recombinant Delta 1 Catenin antibody (clone CTNND1/4383R) at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Negative tissue control. IHC analysis of formalin-fixed, paraffin-embedded human brain stained with CTNND1/4383R at 2ug/ml in PBS for 30min RT. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant Delta 1 Catenin antibody (CTNND1/4383R) as confirmation of integrity and purity.

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NSJ-V9163SAF-100UG100ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the recombinant Delta 1 Catenin antibody should be determined by the researcher.
Description:
Alpha-catenin and beta-catenin bind to the intracellular domain of E-cadherin while p120 catenin (delta-1-catenin) binds E-cadherin at a juxta-membrane site. The complex stabilizes tight junctions. In the cell, delta-1-catenin localized to the E-cadherin/catenins cell adhesion complex, directly associates with cytoplasmic C-terminus of E-cadherin and may similarly interact with other cadherins.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 900-1,000 was used as the immunogen for the recombinant Delta 1 Catenin antibody.
Limitation:
This recombinant Delta 1 Catenin antibody is available for research use only.
Localization:
Cell Surface, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
O60716

Documents