NSJ Bioreagents

ZAP70 Antibody

Product Code:
 
NSJ-V3946SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ZAP70/2047
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the ZAP70 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Flow testing of PFA-fixed Jurkat cells with ZAP70 antibody (clone ZAP70/2047); Red=isotype control, Blue= ZAP70 antibody.
2 / 3
IHC testing of FFPE human tonsil with ZAP70 antibody (clone ZAP70/2047). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 3
SDS-PAGE analysis of purified, BSA-free ZAP70 antibody (clone ZAP70/2047) as confirmation of integrity and purity.
❮❯

Flow testing of PFA-fixed Jurkat cells with ZAP70 antibody (clone ZAP70/2047); Red=isotype control, Blue= ZAP70 antibody.
IHC testing of FFPE human tonsil with ZAP70 antibody (clone ZAP70/2047). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free ZAP70 antibody (clone ZAP70/2047) as confirmation of integrity and purity.

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NSJ-V3946SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA: 2-4ug/ml (order BSA/azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the ZAP70 antibody should be determined by the researcher.
Description:
ZAP70 is a 70kDa protein tyrosine kinase found in T-cells and natural killer cells. Control of this protein translation is via the IgVH gene. ZAP70 protein is expressed in leukemic cells of approximately 25% of chronic lymphocytic leukemia (CLL) cases as well. Anti-ZAP70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (anti-ZAP70 negative) and Ig-unmutated (anti-ZAP70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The anti-ZAP70 positive Ig-unmutated CLL cases have been shown to have a poorer prognosis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A portion of amino acids 247-382 from the human protein was used as the immunogen for the ZAP70 antibody.
Limitation:
This ZAP70 antibody is available for research use only.
Localization:
Cytoplasmic, cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P43403

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