NSJ Bioreagents

MUC1 Antibody / Mucin-1

Product Code:
 
NSJ-V7510SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
VU-4H5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the MUC1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC testing of FFPE human colon carcinoma with MUC1 antibody (clone VU-4H5). HIER: requires steaming of sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
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IHC testing of FFPE human lung carcinoma wi
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IHC testing of FFPE human colon carcinoma with MUC1 antibody (clone VU-4H5). HIER: requires steaming of sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
IHC testing of FFPE human lung carcinoma wi

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NSJ-V7510SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC1 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
MAb VU-4H5 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of MAb VU-4H5 is APDTR as established with epitope fingerprinting. VU-4H5 preferentially binds to under-glycosylated tumor MUC1. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is upregulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. Antibody to MUC1 is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Synthetic glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3-COOH, conjugated to bovine serum albuminn was used as the immunogen for this MUC1 antibody.
Limitation:
This MUC1 antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P15941

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