NSJ Bioreagents

VEGF Antibody

Product Code:
 
NSJ-V8154SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
VG1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the VEGF antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human tonsil with VEGF antibody (clone VG1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free VEGF antibody (clone VG
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IHC staining of FFPE human tonsil with VEGF antibody (clone VG1). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free VEGF antibody (clone VG

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NSJ-V8154SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 2-4ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
This mAb recognizes proteins of 19-22kDa (reducing) and 38kDa-44kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the VEGF antibody.
Limitation:
This VEGF antibody is available for research use only.
Purity:
Protein G affinity chromatography
Uniprot #:
P15692

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