NSJ Bioreagents

Uroplakin 1A Antibody / UPK1A

Product Code:
 
NSJ-V7672SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
UPK1A/2922
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the UPK1A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC staining of FFPE human urinary bladder with UPK1A antibody (clone UPK1A/2922). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human urothelial carcinoma with UPK1A antibody (clone UPK1A/2922). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
3 / 4
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using UPK1A antibody (clone UPK1A/2922). These results demonstrate the foremost specificity of the UPK1A/2922 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
4 / 4
SDS-PAGE analysis of purified, BSA-free UPK1A antibody (clone UPK1A/2922) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human urinary bladder with UPK1A antibody (clone UPK1A/2922). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
IHC staining of FFPE human urothelial carcinoma with UPK1A antibody (clone UPK1A/2922). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.
Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using UPK1A antibody (clone UPK1A/2922). These results demonstrate the foremost specificity of the UPK1A/2922 mAb.<BR>Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
SDS-PAGE analysis of purified, BSA-free UPK1A antibody (clone UPK1A/2922) as confirmation of integrity and purity.

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NSJ-V7672SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is found in the asymmetrical unit membrane (AUM) where it can complex with other transmembrane 4 superfamily proteins. It may play a role in normal bladder epithelial physiology, possibly in regulating membrane permeability of superficial umbrella cells or in stabilizing the apical membrane through AUM/cytoskeletal interactions. The protein may also play a role in tumor suppression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 114-173 were used as the immunogen for the UPK1A antibody.
Limitation:
This UPK1A antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
O00322

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