NSJ Bioreagents

CD2 Antibody

Product Code:
 
NSJ-V2030SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
UMCD2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Functional Study
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store the CD2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Flow cytometry testing of human MOLT-4 cells with CD2 antibody (clone UMCD2); Red=isotype control, Blue= CD2 antibody.
2 / 3
Western blot testing of human Jurkat cells with CD2 antibody (clone UMCD2). Expected molecular weight ~47 kDa.
3 / 3
SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone UMCD2) as confirmation of integrity and purity.
❮❯

Flow cytometry testing of human MOLT-4 cells with CD2 antibody (clone UMCD2); Red=isotype control, Blue= CD2 antibody.
Western blot testing of human Jurkat cells with CD2 antibody (clone UMCD2). Expected molecular weight ~47 kDa.
SDS-PAGE analysis of purified, BSA-free CD2 antibody (clone UMCD2) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V2030SAF-100UG100 ug£534.00
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Further Information

Application Details:
Functional studies (order BSA/sodium azide-free format),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 2-4ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondary antibody and substrates may require the CD2 antibody to be titered up or down for optimal performance.
Description:
CD2 interacts through its amino-terminal domain with the extracellular domain of CD58 (also designated CD2 ligand) to mediate cell adhesion. CD2/CD58 binding can enhance antigen-specific Tcell activation. CD2 is a transmembrane glycoprotein that is expressed on peripheral blood T lymphocytes, NK cells and thymocytes. CD58 is a heavily glycosylated protein with a broad tissue distribution in hematopoietic and other cells, including endothelium. Interaction between CD2 and its counter receptor LFA3 (CD58) on opposing cells optimizes immune system recognition, thereby facilitating communication between helper T lymphocytes and antigen-presenting cells, as well as between cytolytic effectors and target cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Gene ID #:
914
Immunogen:
Human Thymocytes/Sezary T cells were used as the immunogen for this CD2 antibody.
Limitation:
This CD2 antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G purified antibody

Documents