Further Information
Immunohistochemistry (FFPE): 1:200-1:500,Western Blot: 1:5000-1:10000
The stated application concentrations are suggested starting points. Titration of the recombinant AKT antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Akt isoforms are overexpressed in a variety of human tumors, and, at the genomic level, are amplified in gastric adenocarcinomas (Akt1), ovarian (Akt2), pancreatic (Akt2) and breast (Akt2) cancer. The role of Akt3 is less clear, though it appears to be predominantly expressed in the brain.
Akt possesses a protein domain known as a PH domain, or Pleckstrin Homology domain, named after Pleckstrin, the protein in which it was first discovered. This domain binds to phosphoinositides with high affinity. In the case of the PH domain of Akt, it binds either PIP3 (phosphatidylinositol (3,4,5)-trisphosphate, PtdIns(3,4,5)P3) or PIP2 (phosphatidylinositol (3,4)-bisphosphate, PtdIns(3,4)P2).[9] This is useful for control of cellular signaling because the di-phosphorylated phosphoinositide PIP2 is only phosphorylated by the family of enzymes, PI 3-kinases (phosphoinositide 3-kinase or PI3-K), and only upon receipt of chemical messengers which tell the cell to begin the growth process. For example, PI 3-kinases may be activated by a G protein coupled receptor or receptor tyrosine kinase such as the insulin receptor. Once activated, PI 3-kinase phosphorylates PIP2 to form PIP3. [Wiki]
Purified
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
A peptide corresponding to the PH domain of human AKT1/2/3 was used as the immunogen for the recombinant AKT antibody.
This recombinant AKT antibody is available for research use only.
Cytoplasmic, membranous, nuclear
Protein A purified from animal origin-free supernatant
P31749, P31751, Q9Y243