NSJ Bioreagents

FTL Antibody / Ferritin Light Chain

Product Code:
 
NSJ-V8510SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rFTL/1386
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant FTL antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Western blot testing of human kidney lysate with recombinant FTL antibody. Predicted molecular weight: ~20 kDa.
2 / 3
IHC staining of FFPE human kidney with recombinant FTL antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free recombinant FTL antibody as confirmation of integrity and purity.
❮❯

Western blot testing of human kidney lysate with recombinant FTL antibody. Predicted molecular weight: ~20 kDa.
IHC staining of FFPE human kidney with recombinant FTL antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free recombinant FTL antibody as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V8510SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the recombinant FTL antibody should be determined by the researcher.
Description:
Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant full-length human FTL protein was used as the immunogen for the recombinant FTL antibody.
Limitation:
This recombinant FTL antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P02792

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