NSJ Bioreagents

KRT8 Antibody / Cytokeratin 8

Product Code:
 
NSJ-V3560SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rB22.1
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant CK8 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human HCT-116 cell lysate with recombinant CK8 antibody (clone rB22.1).~
2 / 2
SDS-PAGE analysis of purified, BSA-free recombinant CK8 antibody (clone rB22.1) as confirmation of integrity and purity.
❮❯

Western blot testing of human HCT-116 cell lysate with recombinant CK8 antibody (clone rB22.1).~
SDS-PAGE analysis of purified, BSA-free recombinant CK8 antibody (clone rB22.1) as confirmation of integrity and purity.

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NSJ-V3560SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT,Western blot: 1-2ug/ml
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant CK8 antibody to be titered up or down for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A cytoskeletal preparation from human HeLa cells was used as the immunogen for this recombinant CK8 antibody.
Limitation:
This recombinant CK8 antibody is available for research use only.
Localization:
Cytoplasmic
PM_Meta_Key:
Product_Images
PM_Meta_Value:
https://www.nsjbio.com/get_image.php?catalog_no=V3560-100UG&type=image1~Western blot testing of human HCT-116 cell lysate with recombinant CK8 antibody (clone rB22.1).|https://www.nsjbio.com/get_image.php?catalog_no=V3560-100UG&type=image2~SDS-PAGE analysis of purified, BSA-free recombinant CK8 antibody (clone rB22.1) as confirmation of integrity and purity.|https://www.nsjbio.com/get_image.php?catalog_no=V3560-100UG&type=image3~IHC testing of FFPE human colon tissue with recombinant CK8 antibody (clone rB22.1). Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.
Purity:
Protein G affinity chromatography
Uniprot #:
P05787

Documents