NSJ Bioreagents

AKT1 Antibody

Product Code:
 
NSJ-V7745SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
rAKT1/2491
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC staining of FFPE human prostate carcinoma with recombinant AKT1 antibody (clone rAKT1/2491). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
2 / 4
IHC staining of FFPE human colon carcinoma with recombinant AKT1 antibody (clone rAKT1/2491). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
3 / 4
SDS-PAGE analysis of purified, BSA-free recombinant AKT1 antibody (clone rAKT1/2491) as confirmation of integrity and purity.
4 / 4
Western blot testing of human MCF7 cell lysate with AKT1 antibody. Predicted molecular weight ~56 kDa.
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IHC staining of FFPE human prostate carcinoma with recombinant AKT1 antibody (clone rAKT1/2491). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC staining of FFPE human colon carcinoma with recombinant AKT1 antibody (clone rAKT1/2491). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
SDS-PAGE analysis of purified, BSA-free recombinant AKT1 antibody (clone rAKT1/2491) as confirmation of integrity and purity.
Western blot testing of human MCF7 cell lysate with AKT1 antibody. Predicted molecular weight ~56 kDa.

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NSJ-V7745SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 85-189 from the human protein were used as the immunogen for the recombinant AKT1 antibody.
Limitation:
This recombinant AKT1 antibody is available for research use only.
Localization:
Nucleus, plasma membrane, cytoplasm
Purity:
Protein G affinity chromatography
Uniprot #:
P31749

Documents