CD45 Antibody Cocktail (Leukocyte marker)

Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml

Optimal dilution of the antibody should be determined by the researcher.

Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portions of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms. A positive result with this MAb is highly indicative of lymphoid or myeloid origin. Certain types of lymphoid neoplasms may lack CD45 (Hodgkin lymphoma, some T-cell lymphomas, and some leukemias) so its absence does not rule out a hematolymphoid tumor. This antibody is expressed almost exclusively by cells of hematopoietic lineage and is present in most benign and malignant lymphocytes as well as plasma cell precursors.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Human recombinant full-length CD45 protein was used as the immunogen for the CD45 antibody.

This CD45 antibody is available for research use only.

Cell surface, cytoplasmic

Protein G affinity chromatography

P08575