NSJ Bioreagents

Prohibitin Antibody / PHB

Product Code:
 
NSJ-V3517SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
PHB/1881
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Prohibitin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
Western blot testing of human 1) HEK293, 2) Jurkat, 3) HepG2 and 4) U-87 MG cell lysate with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.
2 / 3
IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/1881). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
3 / 3
Western blot testing of human liver lysate with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.
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Western blot testing of human 1) HEK293, 2) Jurkat, 3) HepG2 and 4) U-87 MG cell lysate with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.
IHC testing of FFPE human liver tissue with Prohibitin antibody (clone PHB/1881). Required HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human liver lysate with Prohibitin antibody (clone PHB/1881). Expected molecular weight ~30 kDa.

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NSJ-V3517SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the Prohibitin antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Description:
This antibody recognizes a protein of 30kDa which is identified as Prohibitin (PHB), an evolutionarily conserved protein with homologues found in yeast to man. It is located in the inner membrane of mitochondria. Although PHB mRNA and protein expression occurs throughout the cell cycle, maximum levels are detected during the G1/S phase transition and minimum levels are seen in S phase and the G2/mitosis boundary. Prohibitin is located exclusively in the mitochondria with the highest concentration on the inner membrane. It is an ideal mitochondrial marker. It shows anti-proliferative activity and has been proposed to play a role in normal cell cycle regulation, replicative senescence, cellular immortalization, and tumor suppression.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human partial protein corresponding to amino acids 167-261 was used as the immunogen for the Prohibitin antibody.
Limitation:
This Prohibitin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P35232

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