NSJ Bioreagents

KRT7 Antibody / Cytokeratin 7

Product Code:
 
NSJ-V2654SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
OV-TL12/30
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the Cytokeratin 7 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 4
IHC: Formalin-fixed, paraffin-embedded human lung SCC stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 4
Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody.
4 / 4
SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.
❮❯

IHC: Formalin-fixed, paraffin-embedded ovarian carcinoma stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC: Formalin-fixed, paraffin-embedded human lung SCC stained with Cytokeratin 7 antibody (clone OV-TL12/30). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Immunofluorescent staining of methanol-fixed human HeLa cells with Cytokeratin 7 antibody.
SDS-PAGE analysis of purified, BSA-free Cytokeratin 7 antibody (clone OV-TL12/30) as confirmation of integrity and purity.

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NSJ-V2654SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
The stated application concentrations are suggested starting amounts. Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
3. View the recombinant version of this Cytokeratin 7 antibody.

Description:
CK7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). Expressed in cultured epidermal, bronchial and mesothelial cells. [UniProt]
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
OTN 11 cells (ovarian carcinoma cell line) were used as the immunogen for the Cytokeratin 7 antibody.
Limitation:
This Cytokeratin 7 antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P08729

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