NSJ Bioreagents

NKX3.1 Antibody

Product Code:
 
NSJ-V7569SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2c kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NKX3.1/2576
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the NKX3.1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 3
IHC staining of FFPE human prostate carcinoma with NKX3.1 antibody (clone NKX3.1/2576). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
2 / 3
IHC staining of FFPE human prostate carcinoma with NKX3.1 antibody (clone NKX3.1/2576). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
SDS-PAGE analysis of purified, BSA-free NKX3.1 antibody (clone NKX3.1/2576) as confirmation of integrity and purity.
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IHC staining of FFPE human prostate carcinoma with NKX3.1 antibody (clone NKX3.1/2576). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
IHC staining of FFPE human prostate carcinoma with NKX3.1 antibody (clone NKX3.1/2576). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
SDS-PAGE analysis of purified, BSA-free NKX3.1 antibody (clone NKX3.1/2576) as confirmation of integrity and purity.

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NSJ-V7569SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA (order BSA/sodium azide-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Application Note:
Optimal dilution of the NKX3.1 antibody should be determined by the researcher.
1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
NKX3.1 is a prostate specific gene encoding a transcription factor that plays an important role in normal prostate development and carcinogenesis. It is a prostatic tumor suppressor gene located on chromosome 8p21.2, which frequently undergoes a loss of heterozygosity. NKX3.1 expression is highly restricted in prostate epithelial cells and therefore can be used as a diagnostic biomarker for prostate cancer and other metastatic lesions of prostatic origin. Furthermore, NKX3.1 shows better sensitivity than Prostate Specific Antigen (PSA) for identifying metastatic prostatic adenocarcinoma. This suggests that immunohistochemical staining of NKX3.1, along with other prostate-restricted markers, may be valuable for the definitive determination of prostatic origin in poorly differentiated metastatic carcinomas.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human NKX3.1 protein fragment within amino acids 92-224 was used as the immunogen for the NKX3.1 antibody.
Limitation:
This NKX3.1 antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
Q99801

Documents