CD57 / HNK-1 Antibody

Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Optimal dilution of the CD57 antibody should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Anti-CD57 marks a subset of lymphocytes known as natural killer (NK) cells. Follicular center cell lymphomas often contain many NK cells within the neoplastic follicles. Anti-CD57 also stains neuroendocrine cells and their derived tumors, including carcinoid tumor and medulloblastoma. Anti-CD57 can also be useful in separating type B3 thymoma from thymic carcinoma when combined with a panel that includes antibodies against GLUT1, CD5, and CEA.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Membrane antigen from HSB-2 cells was used as the immunogen for the CD57 antibody.

This CD57 antibody is available for research use only.

Cell surface, cytoplasmic

PEG precipitation

Q9P2W7