NSJ Bioreagents

MMP2 Antibody

Product Code:
 
NSJ-V3247SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MMP2/1501
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Store the MMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of 1) a partial recombinant protein and 2) human U87 cell lysate with MMP2 antibody (clone MMP2/1501). Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form).~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free M
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Western blot testing of 1) a partial recombinant protein and 2) human U87 cell lysate with MMP2 antibody (clone MMP2/1501). Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form).~
SDS-PAGE Analysis of Purified, BSA-Free M

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NSJ-V3247SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml
Application Note:
The stated application concentrations are suggested starting amounts. Titration of the MMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Description:
It recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP2 (also designated type IV collagenase) cleaves collagen types IV,V, VII and X and gelatin type I. Activation of MMP2 secretion requires the Ras signaling pathway.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Amino acids 195-319 were used as the immunogen for the MMP2 antibody.
Limitation:
This MMP2 antibody is available for research use only.
Localization:
Cytoplasmic, cell surface
Purity:
Protein G affinity chromatography
Uniprot #:
P08253

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