NSJ Bioreagents

CD10 Antibody / Neprilysin

Product Code:
 
NSJ-V7954SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
MME/2579
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the CD10 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 4
IHC staining of FFPE human prostate carcinoma with CD10 antibody (clone MME/2579). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE human renal cell carcinoma with CD10 antibody (clone MME/2579). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of human kidney lysate with CD10 antibody (clone MME/2579). Routinely visualized at ~100 kDa.
4 / 4
SDS-PAGE analysis of purified, BSA-free CD10 antibody (clone MME/2579) as confirmation of integrity and purity.
❮❯

IHC staining of FFPE human prostate carcinoma with CD10 antibody (clone MME/2579). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE human renal cell carcinoma with CD10 antibody (clone MME/2579). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Western blot testing of human kidney lysate with CD10 antibody (clone MME/2579). Routinely visualized at ~100 kDa.
SDS-PAGE analysis of purified, BSA-free CD10 antibody (clone MME/2579) as confirmation of integrity and purity.

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CodeSizePrice
NSJ-V7954SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml,Western blot: 1-2ug/ml
Application Note:
Optimal dilution of the antibody should be determined by the researcher.
Description:
Recognizes a 100kDa glycoprotein, identified as CD10, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA). It is a cell surface enzyme with neutral metalloendopeptidase activity, which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt s, and follicular germinal center lymphomas, and on cells from patients with chronic myelocytic leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within adult bone marrow and germinal center B cells within lymphoid tissue. CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant human partial protein (amino acids 583-740) was used as the immunogen for the CD10 antibody.
Limitation:
This CD10 antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Uniprot #:
P08473

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