NSJ Bioreagents

MUC2 Antibody / Mucin 2

Product Code:
 
NSJ-V7308SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
MLP/2970R
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the recombinant MUC2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human colon tissue with recombinant MUC2 antibody (clone MLP/2970R). HIER: boil tissue sections in 10mM Tris-HCl buffer, pH 10, for 10-20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free
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IHC staining of FFPE human colon tissue with recombinant MUC2 antibody (clone MLP/2970R). HIER: boil tissue sections in 10mM Tris-HCl buffer, pH 10, for 10-20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free

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NSJ-V7308SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
Application Note:
Titering of the recombinant MUC2 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Recombinant human protein was used as the immunogen for the recombinant MUC2 antibody. Its epitope has been defined as GTQTP.
Limitation:
This recombinant MUC2 antibody is available for research use only.
Localization:
Cytoplasmic and cell surface
Purity:
Protein A affinity chromatography
Uniprot #:
Q02817

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