NSJ Bioreagents

Plakophilin 1 Antibody

Product Code:
 
NSJ-V3392SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1 kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
10B2
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
Store the Plakophilin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2). Expected molecular weight: 75-83 kDa.~
2 / 2
SDS-PAGE Analysis of Purified, BSA-Free Plakophilin 1 Antibody (clone 10B2). Confirmat
❮❯

Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2). Expected molecular weight: 75-83 kDa.~
SDS-PAGE Analysis of Purified, BSA-Free Plakophilin 1 Antibody (clone 10B2). Confirmat

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NSJ-V3392SAF-100UG100 ug£534.00
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 0.5-1ug/ml
Application Note:
Optimal dilution of the Plakophilin 1 antibody should be determined by the researcher.
Description:
Recognizes a protein of 75kDa, identified as Plakophilin 1 (PKP1). Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. PKP1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress). PKP2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A N-terminal human recombinant protein fragment was used as the immunogen for the Plakophilin 1 antibody. Its epitope maps to between amino acids 1-27 of human PKP1.
Limitation:
This Plakophilin 1 antibody is available for research use only.
Localization:
Cell surface, nuclear
Purity:
Protein G affinity chromatography
Uniprot #:
Q13835

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