NSJ Bioreagents

KDM6A Antibody (Antigen affinity purified)

Product Code:
 
NSJ-RQ6648
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
 
After reconstitution, the KDM6A antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 3
Immunofluorescent staining of FFPE human SiHa cells with KDM6A antibody (Dylight 594-conjugated secondary, red) and Phalloidin-iFluor 488 conjugate (green). HIER: steam section in pH6 citrate buffer for 20 min.
2 / 3
Western blot testing of human 1) MCF7 and 2) HEK293 cell lysate with KDM6A antibody. Predicted molecular weight ~154 kDa.
3 / 3
Flow cytometry testing of human HeLa cells with KDM6A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KDM6A antibody.
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Immunofluorescent staining of FFPE human SiHa cells with KDM6A antibody (Dylight 594-conjugated secondary, red) and Phalloidin-iFluor 488 conjugate (green). HIER: steam section in pH6 citrate buffer for 20 min.
Western blot testing of human 1) MCF7 and 2) HEK293 cell lysate with KDM6A antibody. Predicted molecular weight ~154 kDa.
Flow cytometry testing of human HeLa cells with KDM6A antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= KDM6A antibody.

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CodeSizePrice
NSJ-RQ6648-100ug100ug£535.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunofluorescence (FFPE): 5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Application Note:
Optimal dilution of the KDM6A antibody should be determined by the researcher.
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Lysine-specific demethylase 6A also known as Ubiquitously transcribed tetratricopeptide repeat, X chromosome (UTX), is a protein which in humans is encoded by the KDM6A gene. This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
Recombinant human protein (amino acids A391-A704) was used as the immunogen for the KDM6A antibody.
Limitation:
This KDM6A antibody is available for research use only.
Localization:
Nuclear
Purity:
Antigen affinity purified
Uniprot #:
O15550

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