NSJ Bioreagents

Biotin / Vitamin B7 / Vitamin H Antibody

Product Code:
 
NSJ-V4071SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BTN/8672R
Regulatory Status:
 
RUO
Target Species:
 
Multiple species
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human kidney tissue with Biotin antibody (clone BTN/8672R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human kidney tissue with Biotin antibody (clone BTN/8672R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V4071SAF-100UG100 ug£534.00
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This product comes from: United States.
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Further Information

Application Details:
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Description:
This mAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. It is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody.
Limitation:
This Biotin antibody is available for research use only.
Purity:
Protein A affinity

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