CD20 Antibody

Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT

Recognizes a protein of 30-33kDa, which is identified as CD20. It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. This mAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood and B cell localization in tissues. It reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B-immunoblasts are particularly reactive. It is a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, CD20-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin s disease, particularly of lymphocyte predominant type.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A recombinant partial protein (within amino acids 200-297) from the human protein was used as the immunogen for the recombinant CD20 antibody.

This recombinant CD20 antibody is available for research use only.

Cell Surface

Protein A affinity

P11836