Calponin 1 Antibody

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this mAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A recombinant partial protein sequence (within amino acids 197-297) from the human protein was used as the immunogen for the CNN1 antibody.

This CNN1 antibody is available for research use only.

Cytoplasm

Protein A/G affinity

P51911