NSJ Bioreagents

CD33 Antibody

Product Code:
 
NSJ-V4644SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
SIGLEC3/7612
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the CD33 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Western blot testing of human THP-1 cell lysate with CD33 antibody (clone SIGLEC3/7612). Predicted molecular weight is 40-67 kDa depending on glycosylation level.~
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IHC staining of FFPE human spleen tissue with CD33 antibody (clone SIGLEC3/
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Western blot testing of human THP-1 cell lysate with CD33 antibody (clone SIGLEC3/7612). Predicted molecular weight is 40-67 kDa depending on glycosylation level.~
IHC staining of FFPE human spleen tissue with CD33 antibody (clone SIGLEC3/

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NSJ-V4644SAF-100UG100 ug£534.00
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Further Information

Application Details:
Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
Recognizes a 67kDa glycoprotein, which is identified as CD33. It is a transmembrane protein of the sialic acid-binding immunoglobulin-like lectin (Siglec) family. It belongs to the immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing molecules able of recruiting protein tyrosine phosphatases SHP-1 and SHP-2 to signal assemblies; these ITIMs are also used for ubiquitin-mediated removal of the receptor from the cell surface. CD33 is expressed on cells of myelomonocytic lineage, binds sialic acid residues in N- and O-glycans on cell surfaces, and is a therapeutic target for acute myeloid leukemia. CD33 is expressed on myeloid progenitors, monocytes, granulocytes, dendritic cells and mast cells. It is absent on platelets, lymphocytes, erythrocytes and hematopoietic stem cells.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant fragment of human CD33 protein was used as the immunogen for the CD33 antibody.
Limitation:
This CD33 antibody is available for research use only.
Localization:
Cell Surface, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P20138

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