NSJ Bioreagents

c-Kit Antibody / CD117 / SCFR

Product Code:
 
NSJ-V5051SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C117/8399R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the c-Kit antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human GIST with c-Kit antibody (clone C117/8399R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
2 / 2
SDS-PAGE analysis of purified, BSA-free c-Kit antibo
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IHC staining of FFPE human GIST with c-Kit antibody (clone C117/8399R) at 2ug/ml. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
SDS-PAGE analysis of purified, BSA-free c-Kit antibo

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NSJ-V5051SAF-100UG100 ug£534.00
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Further Information

Application Details:
ELISA: 2-4ug/ml (Order BSA-free format for coating),Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
This mAb recognizes a protein of 145kDa, identified as CD117/p145kit. It is found on a wide variety of tumor cells including follicular and papillary carcinoma of thyroid, adenocarcinomas from endometrium, lung, ovary, pancreas, and breast as well as malignant melanoma, endodermal sinus tumor, and small cell carcinoma. However, anti-CD117 has been particularly useful in differentiating gastrointestinal stromal tumors from Kaposi s sarcoma, tumors of smooth muscle origin, fibromatosis, and neural tumors of the GI tract. Anti-CD117 is also useful in recognizing myeloblasts in bone marrow biopsy and clot section.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant partial protein sequence (within amino acids 50-250) from the human protein was used as the immunogen for the c-Kit antibody.
Limitation:
This c-Kit antibody is available for research use only.
Localization:
Cell surface, Cytoplasm
Purity:
Protein A/G affinity
Uniprot #:
P10721

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