NSJ Bioreagents

SPARC Antibody / Osteonectin

Product Code:
 
NSJ-V5106SAF
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
OSTN/3756
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Osteonectin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPARC / Osteonectin antibody (clone OSTN/3756). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody)
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Analysis of a HuProt(TM) microarray containing more than 19,000 full-length human proteins using SPARC / Osteonectin antibody (clone OSTN/3756). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (in combination with a fluorescently-tagged anti-IgG secondary antibody)

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NSJ-V5106SAF-100UG100 ug£534.00
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.
Format:
Purified
Formulation:
1 mg/ml in 1X PBS; BSA free, sodium azide free
Immunogen:
A recombinant partial protein sequence (within amino acids 1-200) from the human protein was used as the immunogen for the Osteonectin antibody.
Limitation:
This Osteonectin antibody is available for research use only.
Localization:
Secreted
Purity:
Protein A/G affinity
Uniprot #:
P09486

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