Beta Actin Antibody / ACTB

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

All eukaryotic cells express Actin, which often constitutes as much as 50% of total cellular protein. Actin filaments can form both stable and labile structures and are crucial components of microvilli and the contractile apparatus of muscle cells. While lower eukaryotes, such as yeast, have only one Actin gene, higher eukaryotes have several isoforms encoded by a family of genes. At least six types of Actin are present in mammalian tissues and fall into three classes. Alpha-Actin expression is limited to various types of muscle, whereas Beta-Actin and Gamma-Actin are the principle constituents of filaments in other tissues. Members of the small GTPase family regulate the organization of the Actin cytoskeleton. Rho controls the assembly of Actin stress fibers and focal adhesion. Rac regulates Actin filament accumulation at the plasma membrane. Cdc42 stimulates formation of filopodia.

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

A recombinant partial protein sequence (within amino acids 150-350) from the human protein was used as the immunogen for the Beta Actin antibody.

This Beta Actin antibody is available for research use only.

Cytoplasm

Protein A/G affinity

P60709