NSJ Bioreagents

dsDNA Antibody / Double Stranded DNA

Product Code:
 
NSJ-V5319
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rDSD/8266
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Aliquot the Double Stranded DNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
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IHC staining of FFPE human ovarian cancer tissue with Double Stranded DNA antibody (clone rDSD/8266). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~
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IHC staining of FFPE human ovarian cancer tissue with Double Stranded DNA antibody (clone rDSD/8266). Inset: PBS used in place of primary Ab (secondary Ab negative control). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.~

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CodeSizePrice
NSJ-V5319-20UG20 ug£264.00
Quantity:
NSJ-V5319-100UG100 ug£534.00
Quantity:
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Further Information

Application Details:
Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
Description:
This mAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This mAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells.Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Burkitt's cell nuclei were used as the immunogen for the Double Stranded DNA antibody.
Limitation:
This Double Stranded DNA antibody is available for research use only.
Localization:
Nuclear
Purity:
Protein A/G affinity

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