NSJ Bioreagents

Biotin / Vitamin B7 / Vitamin H Antibody

Product Code:
 
NSJ-V5320
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
rBTN/8819
Regulatory Status:
 
RUO
Target Species:
 
Multiple species
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 2
IHC staining of FFPE human kidney tissue with biotinylated Lambda Light Chain antibody probe followed by Biotin antibody (clone rBTN/8819). ~
2 / 2
SDS-PAGE analysis of purified, BSA-free Biotin antibody (clone rBTN/8819) as confirmation of inte
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IHC staining of FFPE human kidney tissue with biotinylated Lambda Light Chain antibody probe followed by Biotin antibody (clone rBTN/8819). ~
SDS-PAGE analysis of purified, BSA-free Biotin antibody (clone rBTN/8819) as confirmation of inte

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CodeSizePrice
NSJ-V5320-20UG20 ug£264.00
Quantity:
NSJ-V5320-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details:
Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 0.5-1ug/ml,Western blot: 0.5-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 minutes at RT
Description:
It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This mAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Format:
Purified
Formulation:
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
Biotinylated sheep immunoglobulin was used as the immunogen for the Biotin antibody.
Limitation:
This Biotin antibody is available for research use only.
Purity:
Protein A/G affinity

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