Absolute Human Circulating Mitochondrial DNA Copy Number Quantification qPCR Assay Kit

Circulating mitochondrial DNA (cir-mtDNA), also called cell-free circulating mitochondrial DNA, refers to small fragments of mitochondrial DNA (mtDNA) released by cells under stress or during various detrimental or pathological conditions. Recently, cir-mtDNA content has gained significant traction as a biomarker for assessing mitochondrial function. It is increasingly employed in the study of changes associated with diseases such as cardiovascular and neurodegenerative disorders, utilizing blood samples and other easy-to-collect specimens such as saliva from large populations. ScienCell's Absolute Human cir-mtDNA Copy Number Quantification qPCR Assay Kit (AHCQ) is designed to quantify the absolute cir-mtDNA copy number of a human plasma/serum sample. The mtDNA primer set recognizes and amplifies one of the most conserved regions on human mtDNA and will not amplify any off-target sequence on nuclear genomic DNA. The reference mitochondrial DNA sample with a known mtDNA copy number per microliter (uL) serves as a reference for cir-mtDNA of target samples. The carefully designed primers ensure: (i) high efficiency for trustworthy quantification; and (ii) no non-specific amplification. mtDNA primer set has been validated by qPCR with melt curve analysis and gel electrophoresis for amplification specificity and by template serial dilution for amplification efficiency. The 2X GoldNStart TaqGreen qPCR Master Mix (Cat #MB6018a-1) is a SYBR®Green dye-based qPCR master mix with a hot-start property. It contains SYBR®Green, dNTPs, Taq DNA polymerase, and an inert gold-color loading indicator in a single tube. The hot-start property achieved through ScienCell's unique chemically modified Taq DNA polymerase provides maximal inhibition of primer dimer formation. The advanced buffer formulation provides superior specificity and efficiency with a wide linear dynamic range. The inert gold-color loading indicator allows for better visualization and tracking of sample loading in qPCR plates or tubes.

ScienCell's Absolute Human cir-mtDNA Copy Number Quantification qPCR Assay Kit (AHCQ) is designed to quantify the absolute cir-mtDNA copy number of a human plasma/serum sample.