NSJ Bioreagents

Phospho-IRAK1 Antibody (S376)

Product Code:
 
NSJ-F54105
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Western Blot (WB)
Storage:
 
Aliquot the phospho-IRAK1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
1 / 1
Western blot analysis of lysates from the human HeLa cell line, untreated or treated with IL-1 beta (20ng/ml) + Calyculin A (100nM), using phospho-IRAK1 antibody (upper) or Tubulin Ab (lower).
❮❯

Western blot analysis of lysates from the human HeLa cell line, untreated or treated with IL-1 beta (20ng/ml) + Calyculin A (100nM), using phospho-IRAK1 antibody (upper) or Tubulin Ab (lower).

No additional charges, what you see is what you pay! *

CodeSizePrice
NSJ-F54105-0.05ML0.05 ml£260.00
Quantity:
NSJ-F54105-0.2ML0.2 ml£502.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Show All

Further Information

Application Details:
WB: 1:1000
Description:
Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor- signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3. [UniProt]
Format:
Antigen affinity purified
Formulation:
In 1X PBS, pH 7.4, with 0.09% sodium azide
Immunogen:
A portion of amino acids 348-381 from human IRAK1 was used as the immunogen for the phospho-IRAK1 antibody.
Purity:
Antigen affinity
Uniprot #:
P51617