NSJ Bioreagents

Light Antibody / Tnfsf14

Product Code:
 
NSJ-RQ7564
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
  • Mouse
  • Rat
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Light antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 4
IHC staining of FFPE mouse spleen tissue with Light antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
2 / 4
IHC staining of FFPE rat spleen tissue with Light antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
3 / 4
Western blot testing of 1) mouse thymus and 2) mouse RAW264.7 cell lysate with Light antibody. Predicted molecular weight ~26 kDa.
4 / 4
Flow cytometry testing of mouse ANA-1 cells with Light antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Light antibody.
❮❯

IHC staining of FFPE mouse spleen tissue with Light antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
IHC staining of FFPE rat spleen tissue with Light antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.
Western blot testing of 1) mouse thymus and 2) mouse RAW264.7 cell lysate with Light antibody. Predicted molecular weight ~26 kDa.
Flow cytometry testing of mouse ANA-1 cells with Light antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Light antibody.

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CodeSizePrice
NSJ-RQ7564-100ug100 ug£535.00
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This product comes from: United States.
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Immunohistochemistry (FFPE): 2-5ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Tumor necrosis factor ligand superfamily member 14 is a protein that in humans is encoded by the TNFSF14 gene. TNFSF14 has also been designated as CD258, as well as LIGHT. It was mapped on chromosome 19p13.3. The protein encoded by this gene is a member of the tumor necrosis factor(TNF) ligand family. This protein may function as a costimulatory factor for the activation of lymphoid cells and as a deterrent to infection by herpesvirus. It has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells. This protein is also reported to prevent tumor necrosis factor alpha mediated apoptosis in primary hepatocyte. Two alternatively spliced transcript variant encoding distinct isoforms have been reported.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant mouse protein (amino acids M1-V239) was used as the immunogen for the Light antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q9QYH9