NSJ Bioreagents

Activated CDC42 kinase 1 Antibody / ACK1

Product Code:
 
NSJ-RQ7565
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Fluorescence-activated cell sorting (FACS)
  • Western Blot (WB)
Storage:
 
After reconstitution, the Activated CDC42 kinase 1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
1 / 2
Flow cytometry testing of human U-2 OS cells with Activated CDC42 kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Activated CDC42 kinase 1 antibody.
2 / 2
Western blot testing of human 1) SH-SY5Y and 2) HepG2 cell lysate with Activated CDC42 kinase 1 antibody. Predicted molecular weight ~114 kDa.
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Flow cytometry testing of human U-2 OS cells with Activated CDC42 kinase 1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Activated CDC42 kinase 1 antibody.
Western blot testing of human 1) SH-SY5Y and 2) HepG2 cell lysate with Activated CDC42 kinase 1 antibody. Predicted molecular weight ~114 kDa.

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CodeSizePrice
NSJ-RQ7565-100ug100 ug£535.00
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Further Information

Application Details:
Western blot: 0.5-1ug/ml,Flow cytometry: 1-3ug/million cells,Direct ELISA: 0.1-0.5ug/ml
Buffer:
Lyophilized from 1X PBS with 2% Trehalose
Description:
Activated CDC42 kinase 1, also known as ACK1, is an enzyme that in humans is encoded by the TNK2 gene. This gene encodes a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47 amino acids C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway. Several alternatively spliced transcript variants have been identified from this gene, but the full-length nature of only two transcript variants has been determined.
Format:
Antigen affinity purified
Formulation:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Immunogen:
E. coli-derived recombinant human protein (amino acids M491-R1038) was used as the immunogen for the Activated CDC42 kinase 1 antibody.
Purity:
Antigen affinity purified
Uniprot #:
Q07912